Journal: The Journal of Veterinary Medical Science

Article Title: Inhibition of transient receptor potential vanilloid type 1 through α 2 adrenergic receptors at peripheral nerve terminals relieves pain

doi: 10.1292/jvms.21-0429

Figure Lengend Snippet: Sequences of primers for RT-PCR

Article Snippet: Subsequently, the sections were incubated with a guinea pig anti-TRPV1 antibody (Merck Millipore corporation, Temecula, CA, USA, Cat# AB5566, Lot# 3135959) diluted to 1:50, a rabbit anti-α 2A antibody (Cusabio Technology, Houston, TX, USA, Cat# CSB-PA007424, Lot# G1229Y) diluted to 1:100 or a rabbit anti-α 2C antibody (Cusabio Technology, Cat# CSB-PA000916, Lot# G1229Y) diluted to 1:100 overnight at 4°C.

Techniques:

Journal: The Journal of Veterinary Medical Science

Article Title: Inhibition of transient receptor potential vanilloid type 1 through α 2 adrenergic receptors at peripheral nerve terminals relieves pain

doi: 10.1292/jvms.21-0429

Figure Lengend Snippet: Subtypes of the α 2 adrenergic receptors detected in dorsal root ganglia. A. The real-time PCR amplification curves are shown in the left panel. A threshold was set for 3, and the Ct values of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α 2 receptors were obtained. The ΔCt values of α 2A (n=8), α 2B (n=8) and α 2C (n=8) receptors against GAPDH were calculated and are shown in the right panel. The columns represent the mean ± the standard error of the mean. B, C. Images of the immunoreactivities of TRPV1, α 2A and α 2C receptors in rat dorsal root ganglia. The TRPV1-, α 2A receptor- and α 2C receptor-like immunoreactivities were visualized by 3,3′-diaminobenzidine. Arrows, positively stained small-sized neurons; arrowheads, positively stained medium-sized neurons.

Article Snippet: Subsequently, the sections were incubated with a guinea pig anti-TRPV1 antibody (Merck Millipore corporation, Temecula, CA, USA, Cat# AB5566, Lot# 3135959) diluted to 1:50, a rabbit anti-α 2A antibody (Cusabio Technology, Houston, TX, USA, Cat# CSB-PA007424, Lot# G1229Y) diluted to 1:100 or a rabbit anti-α 2C antibody (Cusabio Technology, Cat# CSB-PA000916, Lot# G1229Y) diluted to 1:100 overnight at 4°C.

Techniques: Real-time Polymerase Chain Reaction, Amplification, Staining

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: α 1 ‐Adrenergic Receptors Function Within Hetero‐Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C‐X‐C motif) Receptor 4 in Vascular Smooth Muscle Cells

doi: 10.1161/JAHA.117.006575

Figure Lengend Snippet: Validation of anti–chemokine (C‐X‐C motif) receptor 4 ( CXCR 4) antibodies. Human vascular smooth muscle cells were incubated with nontargeting ( NT ) or CXCR 4 small interfering RNA (siRNA) and then used in proximity ligation assays (PLAs) for the detection of CXCR 4 with goat anti‐ CXCR 4 (Abcam Ab1670) and rabbit anti‐ CXCR 4 (Alomone Labs ACR ‐014). A, Representative PLA images showing merged PLA /4′,6‐diamidino‐2‐phenylindole dihydrochloride signals. Ctrl: Omission of primary antibody. Scale bars=10 μm. B, Quantification of PLA signals per cell, as in (A). n=10 images per condition. *: p<0.05 vs. cells incubated with NT siRNA.

Article Snippet: Western blotting with rabbit anti‐ACKR3 (Abcam Ab38089), rabbit anti‐α 1A ‐AR (Abcam Ab137123), rabbit anti‐α 1B ‐AR (Abcam Ab169523), goat anti‐α 1D ‐AR (Santa Cruz SC27099), and mouse anti‐α 2C ‐AR (Abcam Ab167433) in combination with anti‐rabbit, goat, or mouse IgG horseradish peroxidase–linked whole antibody (GE Healthcare and Sigma Aldrich) was performed as previously described.

Techniques: Biomarker Discovery, Incubation, Small Interfering RNA, Ligation